MSK1，a Mitogen and Stress activated protein Kinase，is activated by ERK as well as p38 MAPK in response to growth factors and various cellular stress stimuli. MSK1 resembles RSK by having two kinase domains connected by a regulator linker region. Phosphorylation of RSK1 at Ser 364，Ser381 and Thr574 is critical for RSK1 activity. These sites are analogous to Ser360，Ser376 and Thr581 of MSK1 and maybe important for MSK1 activity as well.
應用類型
Western blot （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Phospho-MSK1（Thr581） detects 90 kDa MSK1 only when phosphorylated at Thr581. The antibody does not cross-react with nonphosphorylated MSK1 or MSK1 phosphorylated at other sites. It does not cross-react with RSK1， RSK2 or RSK3.
免疫原
Synthetic peptide surrounding phospho-Thr581 of human MSK1
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠種屬來源的Phospho-MSK1蛋白發生免疫反應.
保存建議
提供的Phospho-MSK1抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Phospho-MSK1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
