RIP （Receptor Interacting Protein） is a 74 kDa Ser/Thr kinase which interacts with CD95 （Fas/Apo1） receptor and the tumor necrosis factor receptor （TNFR1）. It is a cell death domain adapter protein which can bind to the adapter proteins TRADD，RAID，and TRAF2. RIP contains an N-terminal region with homology to protein kinase，an intermediate domain capable of association with MAPKKK and a C-terminal region containing an intracellular death domain motif. RIP activates both p38 MAP kinase and SAPK families. In vitro，RIP induces apoptosis，as well as SAPK/JNK and NF-kB activation. RIP possesses kinase activity as it autophosphorylates itself on serine and threonine residues.
應用類型
Western blot analysis （2-6 ug/ml）， immunoprecipitation and Immunohistochemistry. However， the optimal conditions should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 645 of human RIP
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的RIP蛋白發生免疫反應.
保存建議
提供的RIP抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該RIP抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
