Three distinct signaling pathways lead to programmed cell death （apoptosis）. The death receptor and mitochondrion pathways are the mains two pathways，in which the key apoptotic proteases caspase-8 and caspase-9，respectively，are involved. The endoplasmic reticulum （ER） stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is located to the ER，but not to the cytoplasm or mitochondrion. Caspase-12 is activated by ER stress，including disruption of ER calcium hemeostasis，and mediates ER stress-induced apoptosis. Caspase-12 is localized to the ER with several proteins that are involved in Alzheimer’s disease including gamma-secretase presenilin and B-amyloid precursor protein （APP）. Caspase-12 mediates cytotoxicity induced by amyloid-B. Caspase-12 is ubiquitously expressed in mouse tissues.
應用類型
Western blotting （0.5-4 ug/ml）. It detects procaspase-12 （55 kDa） and cleavage caspase-12 （36 kDa） on SDS-PAGE immunoblots. However， the optimal conditions should be determined individually.
免疫原
Synthetic peptide corresponding to residues surrounding amino acid 310 of mouse caspase-12
來源宿主
兔
反應性
經檢測驗證，可與小鼠、大鼠種屬來源的Caspase-12蛋白發生免疫反應.
保存建議
提供的Caspase-12抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Caspase-12抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
