Receptor interacting protein including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas，two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but，unlike RIP or RIP2，lacks the c-terminal death or CARD domain. RIP3 binds to RIP and TNFR1，and mediates TNF-R1 mediated apoptosis，and attenuates RIP and TNFR1 induced NFkB activation. Overexpression of RIP3 induces apoptosis and NFkB activation.
應用類型
Western blotting （0.5-4 ug/ml） and in Immunohistochemistry （10-20 ug/ml）. Rat kidney tissue section can be used as a positive control. An approximately 57 kDa band should be detected.
免疫原
Synthetic peptide corresponding to residues surrounding amino acid 476 of mouse RIP3
來源宿主
兔
反應性
經檢測驗證，可與小鼠、大鼠種屬來源的RIP3蛋白發生免疫反應.
保存建議
提供的RIP3抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該RIP3抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
