Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage，a checkpoint kinase ATM phosphorylates and activates Chk2，which in turn phosphorylates and activates p53. Chk2 also phosphorylates and activates BRCA1，the product of a tumor suppressor gene that is mutated in breast and ovarian cancer.
應用類型
Western blotting （0.5-4 ug/ml） and immunofluorescence （5-10 ug/ml）. However， the optimal conditions should be determined individually.
免疫原
Synthetic peptide corresponding to amino acid near the N-terminus of human Chk2
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Chk2蛋白發生免疫反應.
保存建議
提供的Chk2抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Chk2抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
