NF-kB is silenced in the cytoplasm by an inhibitory protein，IkB. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins，IKK1/，IKK2/B，and IKK3/g. External stimuli such TNF- or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified. One of the first gene induced following NF-kB activation is IkB.
應用類型
Western blotting （0.5-4 ug/ml） and immunoprecipitation （1-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects both phosphorylated and non-phosphorylated forms of IkB.
免疫原
Recombinant human IkBa
來源宿主
小鼠
反應性
經檢測驗證，可與人種屬來源的IkBa蛋白發生免疫反應.
保存建議
提供的IkBa抗體產品為100 ug 無色溶液，其濃度為0.25 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS （pH 7.2）緩沖液中.請將該IkBa抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
