Bmf （for Bcl-2-modifying factor） is a novel BH3-only protein recently identified in human and mouse. The BH3 domain in Bmf is required for both binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells，Bmf associates with the dynein light chain 2 （DLC2） component of the myosin V motors and is sequestered by the cell’s actin cytoskeleton. Disruption of the actin cytoskeleton，either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix，triggers release and activation of Bmf，initiating the downstream apoptosis program.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody recognizes ~28 kDa BMF in sample from human， mouse and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 9 of human BMF
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的BMF發生免疫反應.
保存建議
提供的BMF產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該BMF產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
