Livin is a novel member in the IAP （inhibitor of apoptosis） protein family. Livin contains a single baculoviral IAP repeat （BIR） domain and a RING finger domain and has two isoforms termed Livin- and Livin-B. Transfection of Livin protected cells from apoptosis induced by FADD，BAX，RIP，RIP3 and DR6. Livin directly reacts with several caspases including Caspase-3，-7，and –9，and it inhibits the activation of caspase-9 induced by Apaf-1，cytochrome c and ATP.
應用類型
Western blotting （2-6 ug/ml）. However， the optimal dilution conditions should be determined individually. The antibody detects a band at ~33 kDa and a weaker band at 30 kDa， corresponding to Livin and B. Raji cell lysate can be used as a positive control.
免疫原
Synthetic peptide surrounding amino acid near the C-terminus of the long form of human Livin
來源宿主
兔
反應性
經檢測驗證，可與人種屬來源的Livin蛋白發生免疫反應.
保存建議
提供的Livin抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Livin抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
