PUMA （p53 upregulated modulator of apoptosis） is one of the pro-apoptotic Bcl-2 family members which are also transcriptional targets of p53. PUMA gene encodes two BH3 domain-containing proteins termed PUMA- and PUMA-B. PUMA proteins bind Bcl-2，localize to the mitochondria，and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects ~25 kDa （193 a.a.） PUMA alpha and ~16 kDa （131 a.a.） PUMA beta in samples from human， mouse and rat origins.
免疫原
Synthetic peptide surrounding amino acid 177 of human PUMA
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的PUMA/bbc3蛋白發生免疫反應.
保存建議
提供的PUMA/bbc3抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該PUMA/bbc3抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
