Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT，a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation.
應用類型
Western blotting （0.5-4 ug/ml）. Based on researchers feed back， it also works in Immunohistochemistry （10-20 ug/ml）. However， the optimal conditions should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 727 of human Oct-1
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Oct-1蛋白發生免疫反應.
保存建議
提供的Oct-1抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Oct-1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
