Cytochrome c （m.w. 12，500） is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis，prior to caspase activation，DNA fragmentation，and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Mouse small intestine lysate can be used as a positive control. The cytochrome c antibody detects the 12 kDa cytochrome c from mouse and bovine samples.
免疫原
Bovine cytochrome c
來源宿主
兔
反應性
經檢測驗證，可與小鼠、牛種屬來源的Cytochrome c Polyclonal蛋白發生免疫反應.
保存建議
提供的Cytochrome c Polyclonal抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Cytochrome c Polyclonal抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
