Nuclear factor kappa B （NFkB） was identified as a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes. NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit （p50） and a 65 kDa transactivation subunit （p65/RelA）. Both of these subunits exhibit sequence homology to the protooncogene c-Rel. The p50 has an isoform called p49/p52，and both proteins are derived from the amino-terminal of precursor protein p105 and p100. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor，IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide （LPS），pro-inflammatory cytokines （IL-1 & TNF，etc.），and viral infection，IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually.
免疫原
Synthetic peptide corresponding to the N-terminus of human NFkB p50
