The transcription factor ATF-2 （also called CRE-BP1） is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr69 and Thr71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr69 and Thr71 to prolong the half-life of ATF-2. ATF-2 also functions as a histone acetyltransferase （HAT） by specifically acetylating histones H2B and H4 in vitro.
應用類型
Western blotting （1-4 ug/ml）， immunoprecipitation （10 ug/ml）， and Immunohistochemistry （20 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects phosphorylated ATF-2. It recognizes both Thr69/Thr71 dually phosphoralated ATF-2 as well as ATF-2 singly phosphorylated ATF-2 in samples of human， mouse， and rat origins.
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Thr71 of human ATF2
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Phospho-ATF-2蛋白發生免疫反應.
保存建議
提供的Phospho-ATF-2抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Phospho-ATF-2抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
