Transcription factor CREB binds the cAMP response element （CRE） and activates transcription in response to a variety of extracellular signals including neurotransmitters，hormones，membrane depolarization，and growth or neurotrophic factors. Protein kinase A and the calmodulin-dependent protein kinase CaMKII stimulate CREB phosphorylation at Ser133，a key regulatory site controlling transcriptional activity. Phosphorylation at Ser133 is also mediated by p44/42 MAP kinase，p90RSK，p38 MAP kinase and MSK1. CREB appears to play an important role in learning and memory in both flies and mice.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes 43 kDa CREB from samples of human， mouse and rat origins.
免疫原
Synthetic peptide surrounding amino acid 109 of rat CREB
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的CREB Polyclonal蛋白發生免疫反應.
保存建議
提供的CREB Polyclonal抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該CREB Polyclonal抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
