Cadherins comprise a family of Ca-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of cadherins is though to be responsible for binding specificity，transmembrane domains and carboxy terminal domains. The relative short intracellular domains interact with a variety of cytoplasmic proteins，such as B-catenin，to regulate cadherin function.
應用類型
Western blotting （1-2 ug/ml）， immunoprecipitation （10-20 ug/ml）， and Immunohistochemistry （10-20 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects E- and P-cadherin （~95 kDa）. Jurkat cell lysate （Cat.# 2401-100） can be used as a positive control.
免疫原
Synthetic peptide surrounding amino acid 388 of human Cadherin
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠種屬來源的Cadherin蛋白發生免疫反應.
保存建議
提供的Cadherin抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Cadherin抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
