Calpains are cytosolic，calcium-dependent，cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Calpain-1 and 2 consist of a common small subunit and a large variable subunit. Domains in the large subunit include the amino terminal domain I，the proteinase domain II，domain III，and the EF-hand domain IV. Type I calpain requires micromolar amounts of calcium，while type II calpain requires millimolar amounts of calcium.
應用類型
Western blotting （1:200-1000 dilutions）. However， the optimal concentrations should be determined individually. The anti-calpain-2 antibody recognizes the 80 kDa Calpain-2. It does not cross-react with other calpains
免疫原
Synthetic peptide mapping to the C-terminus of the domain III of human Calpain-2 large subuni
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Calpain-2蛋白發生免疫反應.
保存建議
提供的Calpain-2抗體產品為100 ul 無色溶液，其濃度為N/A，溶解于包含有50%甘油， 1% BSA和0.02%硫柳汞的PBS緩沖液中.請將該Calpain-2抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
