CaMKII is a member of calcium/calmodulin-activated kinase，functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase. The activated CaMKII further autophosphorylates itself at Thr286 to render the kinase constitutively active.
應用類型
Western blotting at 1-2 ug/ml. However， the optimal concentrations should be determined individually. The anti-Phospho-CaMKII antibody recognizes only the phosphorylated CaMKII （Thr286） in samples from human， mouse， and rat origins.
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Thr286 of human CaMKII
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Phospho-CaMKII蛋白發生免疫反應.
保存建議
提供的Phospho-CaMKII抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA和0.02%硫柳汞的PBS緩沖液中.請將該Phospho-CaMKII抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
