Etk is a member of the Bruton’s tyrosine kinase family. Etk is expressed in a variety of hematopoietic，epithelial and endothelial cells. It participates in multiple signal transduction pathways. Phosphorylation of tyrosine 566 by Src kinase is required for activation of Etk in vivo. In endothelial and epithelial cells，Etk is regulated by FAK through phosphorylation at tyrosine 40.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control. The antibody recognizes 76 kDa Etk from samples of human， mouse， and rat origins.
免疫原
Synthetic peptide surrounding amino acid 43 of human Etk
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Etk/BMX蛋白發生免疫反應.
保存建議
提供的Etk/BMX抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA和0.02%硫柳汞的PBS緩沖液中.請將該Etk/BMX抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
