Focal adhesion kinase was identified as a 125 kDa substrate for the intrinsic protein tyrosine kinase activity of Src encoded pp60. The deduced amino acid sequence of FAK p125 has shown it to be a cytoplasmic protein tyrosine kinase whose sequence and structural organization are unique as compared to other proteins described to date. Localization of p125 by immunofluorescence suggests that it is primarily found in cellular focal adhesions leading to its designation as focal adhesion kinase （FAK）. FAK is concentrated at the basal edge of only those basal keratinocytes that are actively migrating and rapidly proliferating in repairing burn wounds and is activated and localized to the focal adhesions of spreading keratinocytes in culture. Thus，it has been postulated that FAK may have an important in vivo role in the reepithelialization of human wounds. FAK protein tyrosine kinase activity has also been shown to increase in cells stimulated to grow by use of mitogenic neuropeptides or neurotransmitters acting through G protein coupled receptors.
應用類型
Western blotting （1-2 ug/ml）， immunoprecipitation （5-20 ug/ml）， and immunocytochemistry （10-20 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes 125 kDa FAK of human， mouse， and rat origins.
免疫原
Recombinant human FAK
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的FAK蛋白發生免疫反應.
保存建議
提供的FAK抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA和0.02%硫柳汞的PBS緩沖液中.請將該FAK抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
