Caspase-9 is one of the most important caspases among the caspase family members. Upon induction of apoptosis，cytochrome c released from mitochondria associates with procaspase-9/Apaf-1. The complex processes procaspase-9 into a large subunit （35 kDa or 17 kDa） and small （10 kDa） by self-cleavage at D315. Activated caspase-9 further cleaves other caspase members including caspase-3，one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. In addition to self-cleavage，procaspase-9 can also be cleaved in vivo by caspase-3 at D330. The process served as a positive feedback to amplify the apoptotic signal in caspase activation pathway.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Mouse small intestine and rat kidney tissue lysates can be used as positive controls. The antibody detects 45 kDa procaspase-9， 35/37 kDa cleaved large fragment and the 25 kDa fully cleaved caspase-9.
免疫原
Synthetic peptide surrounding amino acid 345 of mouse caspase-9
來源宿主
兔
反應性
經檢測驗證，可與小鼠、大鼠種屬來源的Caspase-9蛋白發生免疫反應.
保存建議
提供的Caspase-9抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Caspase-9抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
