GADD153 has been described as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. Expression of GADD153 is induced by a variety of cellular stresses，including nutrient deprivation and metabolic perturbations. GADD153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD153 dimers away from “classical” C/EBP binding sites. Thus GADD153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells.
應用類型
Western blotting （0.5-4 ug/ml）， immunoprecipitation （10-20 ug/ml）， and Immunohistochemistry （10-20 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes 25 kDa GADD153 of human， mouse， and rat origins.
免疫原
Synthetic peptide surrounding amino acid 124 of human GADD153
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的GADD153蛋白發生免疫反應.
保存建議
提供的GADD153抗體產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該GADD153抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
