ILKs （Integrin-linked kinases） in combination with integrins and growth factors regulate cell survival，cell cycle，cell-cell adhesion and cell motility. ILK functions as a scaffold bridging the extra-cellular matrix （ECM） and growth factor receptors to the actin cytoskeleton through interactions with integrin，PINCH （which links ILK to the RTKs via Nck2），CH-ILKBP and affixin. ILK phosphorylates several cellular targets including Akt，GSK-3，myosin light chain 2，as well as affixin. These phosphorylation events are key regulatory steps in modulating the activities of the targets.
應用類型
Western blotting （0.5-4 ug/ml）， Immunohistochemistry （10-20 ug/ml， frozen & paraffin）. However， the optimal concentrations should be determined individually. The antibody recognizes 59 kDa of ILK1 from samples of human， mouse， and rat origins. Reactivity to other species has not been determined.
免疫原
Synthetic peptide corresponding to the C-terminus of human ILK-1
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的ILK1蛋白 發生免疫反應.
保存建議
提供的ILK1抗體 產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該ILK1抗體 產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
