IRS-1 （Insulin receptor substrate-1） is phosphorylated in response to stimulation of cells by insulin. Upon ligand-induced phosphorylation，IRS-1 immediately associates with a series of SH2 domain-containing signaling intermediates such as PI3-kinase，GRB2，Syp and Nck. The level of diversity in structure and function of the SH2-containing proteins which binds activated IRS-1 is important for responsiveness to insulin. IRS phosphorylation elicits the growth and metablic effects of insulin growth factors and insulin by allowing glucose uptake，glycogen synthesis and mitogenic activity. IRS-1 migrates between 170 and 185 kDa and is expressed in most cell types.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually
免疫原
Synthetic peptide mapping to the C-terminus of human IRS-1
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的IRS-1蛋白發生免疫反應.
保存建議
提供的IRS-1抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該IRS-1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
