eNOS （Endothelial nitric-oxide synthase） is an important enzyme in the cardiovascular system. eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495. Several protein kinases including Akt/PKB，PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli. In contrast，bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody recognizes 140 kDa eNOS of human， mouse， and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 602 of rat eNOS
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的eNOS蛋白 發生免疫反應.
保存建議
提供的eNOS抗體 產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該eNOS抗體 產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
