MLK3 （Mixed lineage kinase 3） is a serine/threonine kinase that has an amino-terminal SH3 domain followed by the kinase domain and two leucine zippers，a cdc42/Rac1 binding （CRIB） domain and several other domains/motifs at the carboxy-terminal region. MLK3 functions as a MAPKKK of the SAPK/JNK stress pathway by directly phosphorylating SEK1/MKK4 and MKK7，although it is controversial whether MLK3 is involved in p38 stress pathway activation. MLK3 also functions as an IkB kinase and mediates the activation of the transcriptional factor NF-kB stimulated by CD3/CD28，suggesting a role for MLK3 in immune and inflammatory responses.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes 92 kDa MLK3 of human， mouse， and rat origins. It does not cross-react with MLK1， MLK2 and other mixed lineage kinases.
免疫原
Synthetic peptide mapping to the C-terminus of human MLK3.
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的MLK3蛋白發生免疫反應.
保存建議
提供的MLK3抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA和0.02%硫柳汞的PBS緩沖液中.請將該MLK3抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
