LIM kinases （LIMK1 and LIMK2） are serine/threnine kinases that have two zinc finger motifs，known as LIM motifs in their N-terminal regulatory domain. LIM kinases are involved in actin cytoskeleton regulation through Rho-family GTPases and downstream kinases PAKs and ROCK. PAK1 and ROCK phosphorylate LIMK1 and LIMK2，which increases the activity of the kinases. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the N-terminus of Cofilin.
應用類型
Western blotting （1-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects 70 kDa LIMK1 protein. It does not cross-react with LIMK2.
免疫原
Synthetic peptide corresponding to the C-terminus of human LIMK1
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的LIMK1蛋白發生免疫反應.
保存建議
提供的LIMK1抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS （pH 7.2）緩沖液中.請將該LIMK1抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
