MAP kinase-activated protein kinase-2 （MAPKAPK-2），also known as p45 hsp27 kinase，is a 45-54 kDa serine-threonine protein kinase. MAPKAPK-2 is rapidly phosphorylated and activated in response to stress，cytokines，and chemotactic factors. One of the major substrates of MAPKAPK-2 is hsp27，which stimulates actin polymerization in order to facilitate recovery from destruction of cytoskeleton during cellular stresses. Two isoforms are produced due to alternative splicing of the same gene which differs in their C-terminals. This antibody recognizes both isoforms.
應用類型
Western blotting （1 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects phosphorylated MAPKAPK-2 （Thr334） of human and mouse origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide corresponding to residues surrounding Thr334 of human MAPKAPK-2
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠種屬來源的Phospho-MAPKAPK-2蛋白發生免疫反應.
保存建議
提供的Phospho-MAPKAPK-2抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS （pH 7.2）緩沖液中.請將該Phospho-MAPKAPK-2抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
