Both p44 and p42 MAP kinases （Erk1 and Erk2） function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine （202 and 204 of human MAP kinase [Erk1] or 183 and 185 of rat Erk2） at the sequence T*EY* by a single upstream MAP kinase kinase （MEK）. Both kinases are known to weakly autophosphorylate on tyrosine.
應用類型
Western blotting （0.5-4ug/ml）， immunoprecipitation（10-20 ug/ml），and Immunohistochemistry （10-20 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes Phosphorylated p42 and p44 （Erk1 and Erk2）.
免疫原
Synthetic phospho-peptide surrounding Thr202/Tyr204 of human P44MAP kinase
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Phospho-Erk1/2蛋白發生免疫反應.
保存建議
提供的Phospho-Erk1/2抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA和0.02%硫柳汞的PBS緩沖液中.請將該Phospho-Erk1/2抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
