The PAK （p21-activated kinase） family of serine/threonine kinases plays an important role in multiple cellular processes，including cytoskeletal reorganization，MAPK signaling，apoptotic signaling，etc. Binding of Rac/cdc42 to the CRIB （or PBD） domain at the N-terminal region of PAK causes autophosphorylation and conformational change of PAK. Phosphorylation of Ser21 of PAK1 or Ser20 of PAK2 regulates its binding with the adaptor protein Nck.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. Jurkat cell lysate （Cat.# 2601-100） can be used as a positive control. The antibody recognizes 60 kDa Pak2 of human， mouse， rat， and rabbit origins. The antibody does not cross-react with Pak1， Pak3， and Pak4-6. Reactivity to other species has not been tested.
免疫原
Synthetic peptide corresponding to residues surrounding amino acid 5 of human Pak2
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠、兔種屬來源的PAK2蛋白發生免疫反應.
保存建議
提供的PAK2抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該PAK2抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
