The PAK （p21-activated kinase） family of serine/threonine kinases plays an important role in multiple cellular processes，including cytoskeletal reorganization，MAPK signaling，apoptotic signaling，etc. Several mechanisms that induce PAK activation have been reported. Binding of Rac/cdc42 to the CRIB （or PBD） domain at the N-terminal region of PAK causes autophosphorylation and conformational change of PAK. More recently identified members PAK4，PAK5 and PAK6 have lower sequence similarity with PAK1-3 in the regulatory N-terminal region. It has been demonstrated that phosphorylation of serine 474 of PAK4 may play a pivotal role in the activity and function of PAK4 kinase
應用類型
Western blot analysis （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody recognizes ~60-70 kDa Pak4 in samples of human， mouse， and rat origins. The antibody does not cross-react with other Paks. Reactivity to other species has not been tested.
免疫原
Synthetic peptide corresponding to the N-terminus of human Pak4
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠、倉鼠種屬來源的PAK4蛋白發生免疫反應.
保存建議
提供的PAK4抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）緩沖液中.請將該PAK4抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
