Five ionomycin-inducible complementary cDNAs，designated PAR1，2，3，4 and 5，have been isolated from the prostate cancer cell line AT-3. Nucleotide sequencing identified PAR1 as the rat homolog of monkeyP-1，PAR2 as the injury-inducible gene HB-EGF，and PAR3 as the serum-induced gene cyr-61. PAR4 and PAR5 sequences were not found to correspond to any previously described proteins. PAR4 （prostate apoptosis response-4） is specifically expressed by cells entering apoptosis and is not inducible by growth factor stimulation，oxidative stress and necrosis，or growth arrest. The PAR4 gene encodes a 38 kDa protein with a putative nuclear localization signal and carboxy terminal leucine zipper.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. The antibody recognizes PAR4 in samples from of human， mouse， and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 327 of human Par4
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠、狗、馬種屬來源的PAR4蛋白發生免疫反應.
保存建議
提供的PAR4抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該PAR4抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
