PDK1 (Phosphoinositide-dependent protein kinase 1) plays an important role in cell signaling pathways and is involved in regulation of a wide variety of processes,including cell proliferation,differentiation and apoptosis.
應用類型
Western blotting (0.5-4 ug/ml), immunoprecipitation (10-20 ug/ml), and Immunohistochemistry (15-25 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~60 kDa PDK1 of human, mouse, and rat origins. A ~33 kDa cleavage fragment can also been recognized. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 542 of human PDK1
來源宿主
兔
反應性
經檢測驗證,可與人、小鼠、大鼠種屬來源的PDK1蛋白發生免疫反應.
保存建議
提供的PDK1抗體產品為100 ug 無色溶液,其濃度為0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該PDK1抗體產品置于-20 ℃冷凍保存,建議分裝使用以避免反復凍融.