PKC （Protein kinase C） is a calcium-dependent and phospholipid-dependent enzyme that is activated in vivo by the lipid diacylglycerol. PKC has been shown to be involved in signal transduction and various aspects of neoplastic transformation. PKC contains an amino terminal regulatory domain and a carboxy terminal catalytic domain joined by a hinge region. Cleavage of protein kinase C in vitro results in the generation of two functional fragments corresponding to these two domains.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes 60-75 kDa PKC Bisoforms from samples of human， mouse， rat， pig and bovine origins. A 40 kDa band can also be detected， presumably to be a shorter form of PKC isoforms. Reactivity to other species has not been tested.
免疫原
Synthetic peptide corresponding to the C-terminus of mouse PKC B1
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的PKC蛋白發生免疫反應.
保存建議
提供的PKC抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該PKC抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
