PKC （Protein kinase C） is a calcium-dependent and phospholipid-dependent enzyme that is activated in vivo by the lipid diacylglycerol. PKC has been shown to be involved in signal transduction and various aspects of neoplastic transformation. PKC contains an amino terminal regulatory domain and a carboxy terminal catalytic domain joined by a hinge region. Cleavage of protein kinase C in vitro results in the generation of two functional fragments corresponding to these two domains.
應用類型
The antibody can be used in Western blotting （1-2 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes several Phosphorylated PKC （Ser660） isoforms of human， mouse， and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic phospho-peptide surrounding amino acid Ser660 of human PKC beta II
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Phospho-PKC蛋白 發生免疫反應.
保存建議
提供的Phospho-PKC抗體 產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA和0.02% 疊氮鈉的PBS緩沖液中.請將該Phospho-PKC抗體 產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
