Smad proteins，the mammalian homologs of the Drosophila Mothers against dpp （Mad），have been implicated as downstream effectors of TGFB/BMP signaling. Smad1，Smad5，and Smad8 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF-B and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFB signaling by interfering with TGFB-mediated phosphorylation of other Smad family members.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes ~68 kDa Smad4 from samples of of human， mouse， and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide corresponding to amino acids residues around 281 of human Smad4
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Smad4蛋白發生免疫反應.
保存建議
提供的Smad4抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該Smad4抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
