Smad proteins，the mammalian homologs of the Drosophila Mothers against dpp （Mad），have been implicated as downstream effectors of TGFB/BMP signaling. Smad1，Smad5，and Smad8 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF-B and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFB signaling by interfering with TGFB-mediated phosphorylation of other Smad family members.
應用類型
Western blotting （1-4 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes ~60 kDa Smad5 of human， mouse， and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide corresponding to amino acids residues around 252 of human Smad5
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Smad5蛋白發生免疫反應.
保存建議
提供的Smad5抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA和0.02% 疊氮鈉的PBS緩沖液中.請將該Smad5抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
