Membrane receptor signaling by various ligands induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN- and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6，it has been shown that Stat3B appears to be activated by both while Stat3 is activated by EGF，but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 is activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.
應用類型
Western blotting （0.5-4 ug/ml）， However， the optimal concentrations should be determined individually. The antibody recognizes ~113 kDa of Stat2 from samples of human， mouse and rat origins. Reactivity to other species has not been determined.
免疫原
Synthetic peptide surrounding amino acid 615 of rat Stat2
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Stat2蛋白發生免疫反應.
保存建議
提供的Stat2抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該Stat2抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
