Membrane receptor signaling by various ligands induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN- and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6，it has been shown that Stat3B appears to be activated by both while Stat3 is activated by EGF，but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 is activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes ~97 kDa Stat3 in samples from human， mouse， and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 73 of rat Stat3
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Stat3蛋白 發生免疫反應.
保存建議
提供的Stat3抗體 產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該Stat3抗體 產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
