Rab5 is a 24 kDa GTP-binding protein that is localized to the cytoplasmic side of the plasma membrane，clathrin-coated vesicles，and early endosomes. Rab5 is believed to regulate vesicle fusion through a cycle of GDP/GTP exchange and GTP hydrolysis. The different guanine nucleotide binding states of rab5 is postulated to affect its ability to associate or dissociate with membranes during endocytotic membrane traffic. Its GTP-bound form，which represents the active form of Rab5，associates with membrane and regulates vesicle docking and fusion.
應用類型
Western blotting （0.5-4 ug/ml）， immunoprecipitation and immunhistochemistry. However， the optimal concentrations should be determined individually. The antibody recognizes 26 kDa Rab5 of human， mouse， monkey， bovine and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 185 of human Rab5
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠、猴、牛種屬來源的Rab5蛋白發生免疫反應.
保存建議
提供的Rab5抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該Rab5抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
