Rad17 is an essential component of cell cycle checkpoints activated by DNA damage and disruption of DNA replication. DNA lesions such as single- and double-strand breaks，inter- and intra-strand cross-links，and base modifications hamper transcription and replication，resulting in cell cycle arrest，apoptosis，and mutagenesis. Phosphorylation of Rad17 at Ser635 and Ser645 is a critical early event during checkpoint signaling in DNA damaged cells.
應用類型
Western blotting （0.5-4 ug/ml）. However， the optimal concentrations should be determined individually. The antibody recognizes the mature forms of Rad17 （~55 kDa and ~45 kDa） of human， mouse and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 645 of human Rad17
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Rad17蛋白 發生免疫反應.
保存建議
提供的Rad17抗體 產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該Rad17抗體 產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
