Methylation of DNA at cytosine residues plays an important role in regulation of gene expression，genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date，3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1，Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. Dnmt2 contains all the sequence motifs diagnostic of DNA （cytosine-5）-methyltransferases but appears to lack the large N-terminal regulatory domain common to other eukaryotic methyltransferases.
應用類型
Western blotting （0.5-4 ug/ml） and immunofluorescence. However， the optimal concentrations should be determined individually. The antibody recognizes 42 kDa Dnmt 2 of human and mouse origins. Mouse testes tissue lysate can be use as a positive control.
免疫原
Synthetic peptides surrounding amino acid 42 and 364 of mouse Dnmt2
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠種屬來源的Dnmt2蛋白發生免疫反應.
保存建議
提供的Dnmt2抗體產品為100 ug 無色溶液，其濃度為0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該Dnmt2抗體產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
