Heat shock proteins （HSPs） are ubiquitously expressed in all organisms. HSP47 is a molecule expressed constitutively in cells that synthesizes collagen. HSP47 is a procollagen-specific molecular chaperon that resides in the endoplasmic reticulum of procollagen-producing cells and is essential for secretion of procollagen from cells. Significantly，HSP47 plays a vital role in folding and assembling collagen. HSP47 is also known as colligin 1，serpinh1，collagen-binding protein 1 （CBP1） and gp46. The human HSP 47 gene encodes a 47 kDa protein.
應用類型
Western blotting （0.5-4 ug/ml）， immunoprecipitation （10-20 ug/ml） and Immunohistochemistry （10-20 ug/ml） in frozen or paraffin embedded tissues. However， the optimal concentrations should be determined individually. The antibody recognizes 47 kDa Hsp47 of human， mouse， rat， and chick origins. Mouse small intestine tissue lysate （Cat.# 2601-100） can be used as a positive control.
免疫原
Synthetic peptide surrounding amino acid 407 of human HSP47
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠、雞種屬來源的Hsp47蛋白 發生免疫反應.
保存建議
提供的Hsp47抗體 產品為100 ug 無色溶液，其濃度為0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該Hsp47抗體 產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
