- 中文名稱
Measles Virus (MV) Antibody (IgM) BioAssay Qualitative ELISA Kit (Human)
- 英文名字
- Measles Virus (MV) Antibody (IgM) BioAssay Qualitative ELISA Kit (Human)
- 供應商
- USBiological
- 產品貨號
- 351665
- 產品報價
- ￥1/48Tests ￥1/96Tests ￥1/24Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- The Measles Virus (MV) Antibody (IgM) BioAssay ELISA Kit (Human) is for the qualitative determination of MV antibody (IgM) concentration in human serum. This kit is intended for research use only and may not be used for therapeutic or diagnostic applicationsPrinciple of the Test:This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgM conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human MV antibody (IgM) bound in the initial step. The color development is stopped and the intensity of the color is measured.Kit Components:351665A: Microtiter Strips, 1x96 wells. Pre-coated.351665B: Negative Control, 1x300ul351665C: Positive Control, 1x300ul351665D: Sample Diluent, 1x12ml351665E: HRP Conjugate, 1x12ml351665F: Wash Buffer, 30X, 1x30ml351665G: Substrate A, 1x6ml351665H: Substrate B, 1x6ml351665J: Stop Solution, 1x6mlStorage and Stability:Store kit at 4°C. The unopened kit is stable for 6 months after receipt. Once opened, the kit is stable for up to 2 weeks at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Sample Collection and Storage:Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at RT or overnight at 4°C before centrifugation for 15 minutes at 1000xg. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.Serum samples require a 40-fold dilution into normal saline before test. The suggested 40-fold dilution can be achieved by adding 10ul sample to 390ul of normal saline.Assay Procedure:Bring all reagents and samples to RT before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and controls be assayed in duplicate.1. Prepare all reagents, and *samples as directed in the previous sections. 2. Determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc; store unused wells at 4°C. 3. Set a Blank well with 100ul of Sample Diluent.4. Add 100ul Sample Diluent to per well. Then add 10ul of Negative Control, Positive Control or Sample per well.5. Cover with the adhesive strip provided. Incubate for 45 minutes at 37°C.6. Aspirate each well and wash, repeating the process three times for a total of four washes. Wash by filling each well with 200ul of 1X Wash Buffer using a squirt bottle, multi-channel pipette, manifold dispenser or autowasher, and let it stand for 30 seconds; complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining 1X Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.7. Add 100ul of HRP Conjugate to each well (not to Blank!). Cover the microtiter plate with the adhesive strip. Incubate for 30 minutes at 37°C.8. Repeat the aspiration/wash process for four times as in step 6.9. Add 50ul of Substrate A and 50ul Substrate B to each well. Incubate for 10 minutes at 37°C. Protect from light.10. Add 50ul of Stop Solution to each well, gently tap the plate to ensure thorough mixing.11. Determine the optical density of each well within 10 minutes using a microplate reader set to 450nm. Take blank well as zero.*Samples may require dilution. Contact:United States Biological4 Technology WaySalem, MA 01970www.usbio.netTech Support:?tech@usbio.netCustomer Service: service@usbio.netTel:?781-639-5092 ??800-520-3011Fax:?978-594-8052
- 產品特點
- 針對Measles Virus (MV) Antibody (IgM) BioAssay Qualitative ELISA Kit (Human)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Measles Virus (MV) Antibody (IgM) BioAssay Qualitative ELISA Kit (Human)產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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