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中文名稱

Plasminogen (PLG) BioAssay ELISA Kit (Mouse)

英文名字
Plasminogen (PLG) BioAssay ELISA Kit (Mouse)
供應商
USBiological
產品貨號
351682
產品報價
¥1/48Tests   ¥1/96Tests   ¥1/24Tests
產品說明書
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購買方式
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產品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理,是行業中少有的致力于服務客戶,幫助客戶,且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應,專業的產品和解決方案支持,穩健快捷的交貨周期,優質放心的售后服務。我們致力于為您提供有價值的產品和服務,在意您的成功!
產品描述
Plasminogen (PLG), BioAssay ELISA Kit (Mouse) is a competitive ELISA intended for the quantitative determination of PLG concentration in mouse serum, plasma and tissue homogenates.Sensitivity:<15.6ng/mlRange:62.5-4000ng/mlPrecision:Intra-assay CV: <8%Inter-assay CV: <10%Principle of the Assay:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PLG. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated anti-PLG antibody. A competitive inhibition reaction is launched between pre-coated PLG and PLG in the standards or samples. A substrate solution is added to the wells; the color development is stopped and the intensity of the color is measured. The intensity of the color is inversely proportional to the amount of PLG in the samples.Kit Components:227665A: Microtiter Strips (12 x 8 microwells). Pre-coated with PLG; ready to use. 227665B: Standard (Lyophilized), 2x1 vials227665C: HRP Conjugate (100X Concentrate), 1x60ul227665D: HRP Conjugate Diluent, 1x10ml227665E: Sample Diluent 2x20ml227665F: Wash Buffer (25X Concentrate), 1x20ml227665G: TMB Substrate, 1x10ml227665H: Stop Solution, 1x10ml227665J: Wash Buffer (25X Concentrate), 1x20mlStorage and Stability:Unopened kit: Store at 4°C. Unused kit is stable for 6 months after receipt. Opened Kit: Once opened, the following kit components are stable for up to 1 month at 4°C providing this is within the expiration date of the kit: HRP conjugate diluent, Sample diluent, Wash buffer, TMB substrate and Stop solution. The coated plate, standard and HRP conjugate may be stored at 4°C for up to one month; store the standard and HRP conjugate at -20°C if the kit will not be used within one month. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Summary:1. Prepare reagents, working standards and samples as instructed.2. Set a Blank well without any solution3. Add 50ul Standard or sample to each well.4. Add 50ul HRP Conjugate to each well (do not add any to the blank well).5. Incubate for 60 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul TMB substrate to each well8. Incubate for 20 minutes at 37°C. Protect from light12. Add 50ul Stop Solution to each well. Read at 450nm within 5 minutes.
產品特點
針對Plasminogen (PLG) BioAssay ELISA Kit (Mouse)該產品的特點優勢,歡迎查閱官網提供的產品說明書。
保存建議
建議收到Plasminogen (PLG) BioAssay ELISA Kit (Mouse)產品后將其置于4°C/-20°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品,為科研用戶提供了諸多超值選擇。
注意
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