MAP kinase kinase 1 （Mek1），also known as mitogen activated protein kinase kinase，MAP kinase kinase，ERK kinase 1 or microtubule-associated protein 2 kinase，is a 43 kDa dual-specificity protein kinase that phosphorylates threonine and tyrosine residues on MAP kinases such as Erk 1 and 2. Mek1 and Mek2 are involved in a diverse array of cellular processes such as stress-activated response，apoptosis，cytokine-induced cell proliferation，and DNA recombination during meiosis. Mek1 and Mek2 are activated by phosphorylation on serine 218 and 222 by serine-threonine kinase，Raf-1，which is part of the p21ras signal transduction pathway.
應用類型
Western blotting （1:200-1000） and immunocytochemistry （1:100-200）. However， the optimal concentrations should be determined individually. The antibody recognizes 45 kDa phospho-Mek1/2 （Ser217/221）. It does not detect the unphosphorylated protein.
免疫原
Synthetic peptide corresponding to amino acids surrounding Ser217/221 of human MEK1/2
來源宿主
兔
反應性
經檢測驗證，可與人、小鼠、大鼠種屬來源的Phospho-Mek1/2蛋白 發生免疫反應.
保存建議
提供的Phospho-Mek1/2抗體 產品為100 ul 無色溶液，其濃度為0.5 mg/ml，溶解于包含有50%甘油， 1% BSA和0.02% 疊氮鈉的PBS緩沖液中.請將該Phospho-Mek1/2抗體 產品置于-20 ℃冷凍保存，建議分裝使用以避免反復凍融.
