- 中文名稱
Glycated Hemoglobin (HbA1c) BioAssay ELISA Kit (Human)
- 英文名字
- Glycated Hemoglobin (HbA1c) BioAssay ELISA Kit (Human)
- 供應商
- USBiological
- 產品貨號
- 351956
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- The Glycated Hemoglobin (HbA1C) BioAssay ELISA Kit (Human) is a competitive inhibition immunoassay for the in vitro quantitative measurement of HbA1c in human plasma, serum and erythrocyte lysates.Detection Range:12.35-1,000ug/mlSensitivity:<4.95ug/mlPrecision:Intra-Assay CV: <10%Inter-Assay CV: <12%Test Principle:This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for HbA1c has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled HbA1c and unlabeled HbA1c (standards or samples) for limited binding sites on the pre-coated antibody. After incubation, the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of HbA1c in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of HbA1c in the sample.Kit Components:*351956A: Microtiter Plate, 96 wells, Pre-coated, ready to use.*351956B: Standard, 2x1vial 351956C: Standard Diluent, 1x20ml*351956D: Detection Reagent A, 1x120ul*351956E: Detection Reagent B, 1x120ul 351956F: Assay Diluent A, 1x12ml351956G: Assay Diluent B, 1x12ml351956H: TMB Substrate, 1x9ml351956K: Stop Solution, 1x6ml351956L: Wash Buffer, 30X, 1x20mlStorage and Stability:Store *351956A, *351956B, *351956D and *351956E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Materials Required But Not Supplied:1. Microplate reader with 450 ± 10nm filter.2. Precision single or multi-channel pipettes and disposable tips.3. Eppendorf Tubes for diluting samples.4. Deionized or distilled water.5. Absorbent paper for blotting the microtiter plate.6. Container for Wash SolutionSample Preparation and Storage:Serum:Use a serum separator tube and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 20 minutes at approximately 1000xg. Assay freshly prepared serum immediately or store samples in aliquots at -20°C or -80°C for later use. Avoid repeated freeze/thaw cycles.Plasma:Collect plasma using EDTA as anticoagulant. Centrifuge samples for 15 minutes at 1000xg at 2-8°C within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquots at -20°C or -80°C for later use. Avoid repeated freeze/thaw cycles.Note: Serum/plasma samples require about 10-fold dilution (e.g., 20ul sample + 180ul PBS). Samples should be diluted using 0.01M PBS, pH 7.0-7.2.Erythrocyte Lysates:1. Centrifuge whole blood for approximately 20 minutes at 1000xg. Remove the supernatant and collect the cells.2. Wash cells three times in cold PBS (0.02M, pH 7.0-7.2).3. Resuspend the cells in ice-cold PBS. Perform a freeze-thaw cycle by freezing the cells at -20°C or lower and then thawing the cells with gentle mixing. Perform the freeze-thaw cycle 3 times.4. Centrifuge for 10 minutes at 5000xg at 2-8°C to remove cellular debris.5. Assay the supernatant immediately or store samples in aliquots at -20°C or -80°C for later use. Avoid repeated freeze/thaw cycles.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.3. Aspirate and wash 3 times.4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.5. Aspirate and wash 5 times.6. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C.7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.
- 產品特點
- 針對Glycated Hemoglobin (HbA1c) BioAssay ELISA Kit (Human)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Glycated Hemoglobin (HbA1c) BioAssay ELISA Kit (Human)產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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