ADAM12, BioAssay ELISA Kit (Mouse) (Disintegrin and Metalloproteinase Domain-containing Protein 12)-ADAM12, BioAssay ELISA Kit (Mouse) (Disintegrin and Metalloproteinase Domain-containing Protein 12)代理/參數/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: ADAM12, BioAssay ELISA Kit (Mouse) (Disintegrin and Metalloproteinase Domain-containing Protein 12)

英文名字: ADAM12, BioAssay ELISA Kit (Mouse) (Disintegrin and Metalloproteinase Domain-containing Protein 12)

供應商: USBiological

產品貨號: 352690

產品報價: ￥1/96 Tests

產品說明書: 點擊查看

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產品描述: ADAM12(Disintegrin and metalloproteinase domain-containing protein 12), also known asmlTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.Sample Type:Cell culture supernates, serum and plasma (heparin)Intended Use:For quantitative detection of mouse ADAM12 in cell culture supernates, serum and plasma(heparin).Sensitivity:<10pg/mlRange:156-10,000pg/mlSpecificity:Natural and recombinant mouse ADAM12Test Principle:This mouse ADAM12 BioAssay ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ADAM12 has been precoated onto 96-well plates. Standards(CHO, R29-S513) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ADAM12 amount of sample captured in plate.Kit Components:96-well plate precoated with mouse ADAM12 antibody, 1Lyophilized recombinant mouse ADAM12 standard, 10ng/tube×2Biotinylated mouse ADAM12 antibody, 130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100)Sample diluent buffer, 30mlAntibody diluent buffer, 12mlABC diluent buffer, 12mlTMB color developing agent, 10mlTMB stop solution, 10mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

產品特點: 針對ADAM12, BioAssay ELISA Kit (Mouse) (Disintegrin and Metalloproteinase Domain-containing Protein 12)該產品的特點優勢，歡迎查閱官網提供的產品說明書。

保存建議: 建議收到ADAM12, BioAssay ELISA Kit (Mouse) (Disintegrin and Metalloproteinase Domain-containing Protein 12)產品后將其置于4°C/-20°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。