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中文名稱

Heme Oxygenase 1, Decycling (HO-1), BioAssay ELISA Kit (Human)

英文名字
Heme Oxygenase 1, Decycling (HO-1), BioAssay ELISA Kit (Human)
供應商
USBiological
產品貨號
352892
產品報價
¥1/96 Tests
產品說明書
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購買方式
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產品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理,是行業中少有的致力于服務客戶,幫助客戶,且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應,專業的產品和解決方案支持,穩健快捷的交貨周期,優質放心的售后服務。我們致力于為您提供有價值的產品和服務,在意您的成功!
產品描述
HMOX1 (heme oxygenase (decycling) 1) is a human gene that encodes for the enzyme heme oxygenase 1. It is localized to chromosome 22. Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.Intended Use:Heme Oxygenase 1, Decycling (HO-1), BioAssay ELISA Kit is a sandwich ELISA for quantitative detection of human HO-1 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).Range:156-10,000pg/mlSensitivity:<10pg/mlSpecificity:Natural and recombinant human HO-1Crossreactivity:There is no detectable cross-reactivity with other relevant proteins.Kit Components:352892A: Microtiter Strips, 1x96 wells352892B: HO-1 Standard, 2x10ng352892C: Anti-human HO-1 (Biotin), 1x130ul (1:100 dilution)USB Cat NoKit Component143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution)143697: Sample Diluent Buffer, 1x30ml143698: Antibody Diluent Buffer, 1x12ml143699: ABC Diluent Buffer, 1x12ml143700: TMB Color Developing Agent, 1x10ml143701: TMB Stop Solution, 2N H2SO4, 1x10mlStorage and Stability:Store *352892B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Assay Summary:1. Add samples and standards and incubate the plate at 37°C for 90 minutes. Do not wash. 2. Add biotinylated antibodies and incubate the plate at 37°C for 60 minutes. Wash plate 3 times with 0.01M TBS or PBS.3. Add ABC working solution and incubate the plate at 37°C for 30 minutes. Wash plate 5 times with 0.01M TBS or PBS.4. Add TMB color developing agent and incubate the plate at 37°C in dark for 25-30 minutes.5. Add TMB stop solution and read.
產品特點
針對Heme Oxygenase 1, Decycling (HO-1), BioAssay ELISA Kit (Human)該產品的特點優勢,歡迎查閱官網提供的產品說明書。
保存建議
建議收到Heme Oxygenase 1, Decycling (HO-1), BioAssay ELISA Kit (Human)產品后將其置于4°C/-20°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品,為科研用戶提供了諸多超值選擇。
注意
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