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中文名稱

IL1R2, BioAssay ELISA Kit (Human) (Interleukin 1 Receptor Type II, CD121b)

英文名字
IL1R2, BioAssay ELISA Kit (Human) (Interleukin 1 Receptor Type II, CD121b)
供應商
USBiological
產品貨號
352936
產品報價
¥1/96 Tests
產品說明書
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產品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理,是行業中少有的致力于服務客戶,幫助客戶,且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應,專業的產品和解決方案支持,穩健快捷的交貨周期,優質放心的售后服務。我們致力于為您提供有價值的產品和服務,在意您的成功!
產品描述
Interleukin 1 receptor, type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is an interleukin receptor. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin-1 receptor family. It is mapped to 2q11.2. IL1R2 binds interleukin alpha(IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. IL1R2 also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. The secreted IL1R2 recruits secreted IL1RAP with high affinity, and this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.Sample Type:Cell culture supernates and serum.Intended Use:For quantitative detection of human IL1R2 in cell culture supernates and serum.Sensitivity:<10pg/mlRange:31.2-2000pg/mlSpecificity:Natural and recombinant human IL1R2Test Principle:This human IL1R2 BioAssay ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL1R2 has been precoated onto 96-well plates. Standards(sf21) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL1R2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL1R2 amount of sample captured in plate.Kit Components:96-well plate precoated with human IL1R2 antibody, 1Lyophilized recombinant human IL1R2 standard, 10ng/tubex2Biotinylated human IL1R2 antibody, 130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex(ABC), 130ul (dilution 1:100)Sample diluent buffer, 30mlAntibody diluent buffer, 12mlABC diluent buffer, 12mlTMB color developing agent, 10mlTMB stop solution, 10mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
產品特點
針對IL1R2, BioAssay ELISA Kit (Human) (Interleukin 1 Receptor Type II, CD121b)該產品的特點優勢,歡迎查閱官網提供的產品說明書。
保存建議
建議收到IL1R2, BioAssay ELISA Kit (Human) (Interleukin 1 Receptor Type II, CD121b)產品后將其置于4°C/-20°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品,為科研用戶提供了諸多超值選擇。
注意
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