MIP-3 alpha, BioAssay ELISA Kit (Mouse) (CCL20, Macrophage Inflammatory Protein 3 alpha, C-C Motif Chemokine 20)-MIP-3 alpha, BioAssay ELISA Kit (Mouse) (CCL20, Macrophage Inflammatory Protein 3 alpha, C-C Motif Chemokine 20)代理/參數/廠家-細胞分析試劑盒-艾美捷科技有限公司

PRODUCT CENTER

細胞分析試劑盒

產品中心

當前位置：首頁 > 產品中心 > 細胞生物學產品 > 細胞分析試劑盒

中文名稱: MIP-3 alpha, BioAssay ELISA Kit (Mouse) (CCL20, Macrophage Inflammatory Protein 3 alpha, C-C Motif Chemokine 20)

英文名字: MIP-3 alpha, BioAssay ELISA Kit (Mouse) (CCL20, Macrophage Inflammatory Protein 3 alpha, C-C Motif Chemokine 20)

供應商: USBiological

產品貨號: 352978

產品報價: ￥1/96 Tests

產品說明書: 點擊查看

購買方式: 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868

產品新聞

背景資料: USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！

產品描述: Macrophage Inflammatory Protein 3 alpha (MIP3alpha), also called Chemokine, cc motif, ligand 20(CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.Sample Type:Cell culture supernates, serum and plasma (heparin, EDTA)Intended Use:For quantitative detection of mouse CCL20 in cell culture supernates, serum and plasma (heparin, EDTA).Sensitivity:<1pg/mlRange:7.8-500pg/mlSpecificity:Natural and recombinant mouse CCL20 Test Principle:This mouse CCL20 BioAssay ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL20 has been precoated onto 96-well plates. Standards(E.coli) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL20 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL20 amount of sample captured in plate.Kit Components:Plate precoated with mouse CCL20 antibody, 1x96-wellLyophilized recombinant mouse CCL20 standard, 10ng/tube, x2Biotinylated mouse CCL20 antibody, 130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex(ABC), 130ul (dilution 1:100)Sample diluent buffer, 30mlAntibody diluent buffer, 12mlABC diluent buffer, 12mlTMB color developing agent, 10mlTMB stop solution, 10mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

產品特點: 針對MIP-3 alpha, BioAssay ELISA Kit (Mouse) (CCL20, Macrophage Inflammatory Protein 3 alpha, C-C Motif Chemokine 20)該產品的特點優勢，歡迎查閱官網提供的產品說明書。

保存建議: 建議收到MIP-3 alpha, BioAssay ELISA Kit (Mouse) (CCL20, Macrophage Inflammatory Protein 3 alpha, C-C Motif Chemokine 20)產品后將其置于4°C/-20°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。